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amino acids 1 81  (Addgene inc)


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    Structured Review

    Addgene inc amino acids 1 81
    Amino Acids 1 81, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amino+acids+1+81/Quas%3AGFPpA%3BBetacrystallin%3ACFP+(Plasmid+%2381181)/pmc11844255-366-12-24
    Average 93 stars, based on 3 article reviews
    amino acids 1 81 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Generated:

    Article Title: NRF2-mediated persistent adaptation of oesophageal adenocarcinoma cells to HER2 inhibition
    Article Snippet: .. Constitutively activated (ca) NRF2 lacking amino acids 1–81 and full length NRF2 cDNAs were generated from the NRF2 template (Addgene #21555), and subcloned into the pLV-EF1aIRES-Puro plasmid (Addgene #85132) using BamHI and EcoRI restriction enzymes. ..

    Article Title: The Golgi complex governs natural killer cell lytic granule positioning to promote directionality in cytotoxicity.
    Article Snippet: YTS cells were also transduced with a GALTmCherry fusion protein containing the amino acids 1–81 from b1,4-galactosyltransferase (pmCherry-N1-GalT was a gift from Lei Lu, Addgene plasmid # 87327)12 or a fusion protein of pHluorin-LAMP1-mApple as previously reported.3 All constructs were generated or subcloned by Epoch Life Science custom cloning services (Sugarland, TX). .. YTS cells were also transduced with a GALTmCherry fusion protein containing the amino acids 1–81 from b1,4-galactosyltransferase (pmCherry-N1-GalT was a gift from Lei Lu, Addgene plasmid # 87327)12 or a fusion protein of pHluorin-LAMP1-mApple as previously reported.3 All constructs were generated or subcloned by Epoch Life Science custom cloning services (Sugarland, TX). .. Cells were stained for flow cytometric analyses as described,19 and 1x106 cells were analyzed using a NovoCyte Penteon flow cytometer (at the Columbia Stem Cell Initiative Flow Cytometry Core Facility); this data were analyzed using FlowJo software.

    Plasmid Preparation:

    Article Title: NRF2-mediated persistent adaptation of oesophageal adenocarcinoma cells to HER2 inhibition
    Article Snippet: .. Constitutively activated (ca) NRF2 lacking amino acids 1–81 and full length NRF2 cDNAs were generated from the NRF2 template (Addgene #21555), and subcloned into the pLV-EF1aIRES-Puro plasmid (Addgene #85132) using BamHI and EcoRI restriction enzymes. ..

    Article Title: The Golgi complex governs natural killer cell lytic granule positioning to promote directionality in cytotoxicity.
    Article Snippet: YTS cells were also transduced with a GALTmCherry fusion protein containing the amino acids 1–81 from b1,4-galactosyltransferase (pmCherry-N1-GalT was a gift from Lei Lu, Addgene plasmid # 87327)12 or a fusion protein of pHluorin-LAMP1-mApple as previously reported.3 All constructs were generated or subcloned by Epoch Life Science custom cloning services (Sugarland, TX). .. YTS cells were also transduced with a GALTmCherry fusion protein containing the amino acids 1–81 from b1,4-galactosyltransferase (pmCherry-N1-GalT was a gift from Lei Lu, Addgene plasmid # 87327)12 or a fusion protein of pHluorin-LAMP1-mApple as previously reported.3 All constructs were generated or subcloned by Epoch Life Science custom cloning services (Sugarland, TX). .. Cells were stained for flow cytometric analyses as described,19 and 1x106 cells were analyzed using a NovoCyte Penteon flow cytometer (at the Columbia Stem Cell Initiative Flow Cytometry Core Facility); this data were analyzed using FlowJo software.

    Article Title: A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes
    Article Snippet: .. To clone GalT-iRFP670, the coding sequence of amino acids 1–81 of GalT was amplified by the PCR and ligated into piRFP670-N1 vector (Addgene plasmid #45457) at Eco RI/ Bam HI sites. .. To clone GFP-ACBD3, the coding sequence of ACBD3 was released from an IMAGE clone (GenBank Accession No. BC045533) by Spe I, end-blunted, digested by Eco RI, and ligated to pEGFP-C2 (Takara Bio), which was prepared by Bam HI digestion, end-blunting, and subsequent Eco RI digestion.

    Article Title: The Golgi complex governs natural killer cell lytic granule positioning to promote directionality in cytotoxicity
    Article Snippet: .. YTS cells were also transduced with a GALT-mCherry fusion protein containing the amino acids 1–81 from β1,4-galactosyltransferase (pmCherry-N1-GalT was a gift from Lei Lu, Addgene plasmid # 87327) or a fusion protein of pHluorin-LAMP1-mApple as previously reported. .. All constructs were generated or subcloned by Epoch Life Science custom cloning services (Sugarland, TX).

    Transduction:

    Article Title: The Golgi complex governs natural killer cell lytic granule positioning to promote directionality in cytotoxicity.
    Article Snippet: YTS cells were also transduced with a GALTmCherry fusion protein containing the amino acids 1–81 from b1,4-galactosyltransferase (pmCherry-N1-GalT was a gift from Lei Lu, Addgene plasmid # 87327)12 or a fusion protein of pHluorin-LAMP1-mApple as previously reported.3 All constructs were generated or subcloned by Epoch Life Science custom cloning services (Sugarland, TX). .. YTS cells were also transduced with a GALTmCherry fusion protein containing the amino acids 1–81 from b1,4-galactosyltransferase (pmCherry-N1-GalT was a gift from Lei Lu, Addgene plasmid # 87327)12 or a fusion protein of pHluorin-LAMP1-mApple as previously reported.3 All constructs were generated or subcloned by Epoch Life Science custom cloning services (Sugarland, TX). .. Cells were stained for flow cytometric analyses as described,19 and 1x106 cells were analyzed using a NovoCyte Penteon flow cytometer (at the Columbia Stem Cell Initiative Flow Cytometry Core Facility); this data were analyzed using FlowJo software.

    Article Title: The Golgi complex governs natural killer cell lytic granule positioning to promote directionality in cytotoxicity
    Article Snippet: .. YTS cells were also transduced with a GALT-mCherry fusion protein containing the amino acids 1–81 from β1,4-galactosyltransferase (pmCherry-N1-GalT was a gift from Lei Lu, Addgene plasmid # 87327) or a fusion protein of pHluorin-LAMP1-mApple as previously reported. .. All constructs were generated or subcloned by Epoch Life Science custom cloning services (Sugarland, TX).

    Construct:

    Article Title: The Golgi complex governs natural killer cell lytic granule positioning to promote directionality in cytotoxicity.
    Article Snippet: YTS cells were also transduced with a GALTmCherry fusion protein containing the amino acids 1–81 from b1,4-galactosyltransferase (pmCherry-N1-GalT was a gift from Lei Lu, Addgene plasmid # 87327)12 or a fusion protein of pHluorin-LAMP1-mApple as previously reported.3 All constructs were generated or subcloned by Epoch Life Science custom cloning services (Sugarland, TX). .. YTS cells were also transduced with a GALTmCherry fusion protein containing the amino acids 1–81 from b1,4-galactosyltransferase (pmCherry-N1-GalT was a gift from Lei Lu, Addgene plasmid # 87327)12 or a fusion protein of pHluorin-LAMP1-mApple as previously reported.3 All constructs were generated or subcloned by Epoch Life Science custom cloning services (Sugarland, TX). .. Cells were stained for flow cytometric analyses as described,19 and 1x106 cells were analyzed using a NovoCyte Penteon flow cytometer (at the Columbia Stem Cell Initiative Flow Cytometry Core Facility); this data were analyzed using FlowJo software.

    Cloning:

    Article Title: The Golgi complex governs natural killer cell lytic granule positioning to promote directionality in cytotoxicity.
    Article Snippet: YTS cells were also transduced with a GALTmCherry fusion protein containing the amino acids 1–81 from b1,4-galactosyltransferase (pmCherry-N1-GalT was a gift from Lei Lu, Addgene plasmid # 87327)12 or a fusion protein of pHluorin-LAMP1-mApple as previously reported.3 All constructs were generated or subcloned by Epoch Life Science custom cloning services (Sugarland, TX). .. YTS cells were also transduced with a GALTmCherry fusion protein containing the amino acids 1–81 from b1,4-galactosyltransferase (pmCherry-N1-GalT was a gift from Lei Lu, Addgene plasmid # 87327)12 or a fusion protein of pHluorin-LAMP1-mApple as previously reported.3 All constructs were generated or subcloned by Epoch Life Science custom cloning services (Sugarland, TX). .. Cells were stained for flow cytometric analyses as described,19 and 1x106 cells were analyzed using a NovoCyte Penteon flow cytometer (at the Columbia Stem Cell Initiative Flow Cytometry Core Facility); this data were analyzed using FlowJo software.

    Sequencing:

    Article Title: A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes
    Article Snippet: .. To clone GalT-iRFP670, the coding sequence of amino acids 1–81 of GalT was amplified by the PCR and ligated into piRFP670-N1 vector (Addgene plasmid #45457) at Eco RI/ Bam HI sites. .. To clone GFP-ACBD3, the coding sequence of ACBD3 was released from an IMAGE clone (GenBank Accession No. BC045533) by Spe I, end-blunted, digested by Eco RI, and ligated to pEGFP-C2 (Takara Bio), which was prepared by Bam HI digestion, end-blunting, and subsequent Eco RI digestion.

    Amplification:

    Article Title: A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes
    Article Snippet: .. To clone GalT-iRFP670, the coding sequence of amino acids 1–81 of GalT was amplified by the PCR and ligated into piRFP670-N1 vector (Addgene plasmid #45457) at Eco RI/ Bam HI sites. .. To clone GFP-ACBD3, the coding sequence of ACBD3 was released from an IMAGE clone (GenBank Accession No. BC045533) by Spe I, end-blunted, digested by Eco RI, and ligated to pEGFP-C2 (Takara Bio), which was prepared by Bam HI digestion, end-blunting, and subsequent Eco RI digestion.

    Polymerase Chain Reaction:

    Article Title: A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes
    Article Snippet: .. To clone GalT-iRFP670, the coding sequence of amino acids 1–81 of GalT was amplified by the PCR and ligated into piRFP670-N1 vector (Addgene plasmid #45457) at Eco RI/ Bam HI sites. .. To clone GFP-ACBD3, the coding sequence of ACBD3 was released from an IMAGE clone (GenBank Accession No. BC045533) by Spe I, end-blunted, digested by Eco RI, and ligated to pEGFP-C2 (Takara Bio), which was prepared by Bam HI digestion, end-blunting, and subsequent Eco RI digestion.



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